Fate of the sblA transcript in Streptomyces lividans and Escherichia coli.
نویسندگان
چکیده
In Streptomyces lividans, the tight temporal regulation of the transient expression of the sblA gene was shown to involve an operator-like sequence located on the sblA transcript. This operator-like structure constitutes a stem-loop structure containing a Shine/Dalgarno-like sequence. Its destruction, by site directed mutagenesis, led to an enhancement of sblA expression. This structure thus plays a negative role in the regulation of sblA expression and might be involved in the regulation of the specific degradation of the sblA transcript. In this issue, the fates of the sblA transcript, in S. lividans and in Escherichia coli, were compared. Analysis of the decay of the sblA transcript revealed that, in both species, the sblA transcript was cleaved just behind the stem-loop structure by an RNAse E-like activity. In E. coli, three discrete products resulting from the cleavage of the full-length transcript by the RNAase E at another site, located 282 nucleotides downstream of the stem-loop structure, were detected whereas only one processed product, corresponding to the 5' end of the gene, was detected in S. lividans. These differences in the mode of degradation of the sblA transcript in S. lividans and E. coli are discussed.
منابع مشابه
Phosphoinositides are involved in control of the glucose-dependent growth resumption that follows the transition phase in Streptomyces lividans.
The interruption of the sblA gene of Streptomyces lividans was previously shown to lead to relief of glucose repression of the normally strongly glucose-repressed alpha-amylase gene. In addition to this relief, an early entry into stationary phase was observed when cells were grown in a minimal medium containing glucose as the main carbon source. In this study, we established that this mutant d...
متن کاملTransformation of Arthrobacter and studies on the transcription of the Arthrobacter ermA gene in Streptomyces lividans and Escherichia coli.
We report the development of a plasmid-mediated transformation system for Arthrobacter sp. NRRLB3381, using the Streptomyces cloning vector pIJ702. Our procedure gives a transformation frequency of 10(3)/micrograms of plasmid DNA. In addition we have explored the expression of the Arthrobacter ermA gene in Streptomyces lividans and Escherichia coli, and shown that the ermA promoter is recognize...
متن کاملDisruption of sblA in Streptomyces lividans permits expression of a heterologous alpha-amylase gene in the presence of glucose.
In a transposition mutant of Streptomyces lividans TK24, the usually glucose-repressible expression of a heterologous alpha-amylase gene (aml) became resistant to glucose repression. The transposon had inserted into an ORF called sblA which encodes a 274 aa product sharing significant sequence similarities with various phosphatases that act on small phosphorylated substrates. sblA was transcrib...
متن کاملCloning and expression of a nitroaryl reductase gene from Streptomyces aminophilus strain MCMB411 in E. coli JM109 and Streptomyces lividans TK64.
A partial genomic library was prepared in E. coli JM109 using pBR322 as vector and 2.4 kb Sau 3A I chromosomal fragment, encoding a nitroaryl reductase (nbr A) gene, from Streptomyces aminophilus strain MCMB 411. From the library, 2.4 kb fragment was recloned in E. coli JM109 and S. lividans TK64 using pUC18 and pIJ702 as vectors respectively. The recombinant plasmids pSD103 and pSD105 expresse...
متن کاملSecretory production of tetrameric native full-length streptavidin with thermostability using Streptomyces lividans as a host
BACKGROUND Streptavidin is a tetrameric protein derived from Streptomyces avidinii, and has tight and specific biotin binding affinity. Applications of the streptavidin-biotin system have been widely studied. Streptavidin is generally produced using protein expression in Escherichia coli. In the present study, the secretory production of streptavidin was carried out using Streptomyces lividans ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- FEMS microbiology letters
دوره 276 1 شماره
صفحات -
تاریخ انتشار 2007